DEPHOSPHORYLATIONOFLINEARIZEDDNA-资讯-分析测试百科网wiki版

来自 : 发布时间：2024-12-23

DEPHOSPHORYLATION OF LINEARIZED DNAALKALINE PHOSPATASE(CIP) DIGESTIONDigestion of protruding 5 -ends1. Precipitate digested DNA and resuspend in a 100ml of 1X CIP Buffer.2. Add 1U CIP for 100pmol 5 -ends.2g linearized 5kb plasmid Å 1.4pmol 5 -ends3. Incubate 30 @ 37oC.4. Add 1ml 0.5M EDTA (to 5mM).5. Phenol-Sevag extract to remove all the enzyme and NaOAc/EtOH ppt.6. Resuspend in ddH2O or TE-4 @ Å100......阅读全文 DNA的酶学操作 DNA的酶学操作DNA Modifying Enzymes(Michael Blaber)Introduction to bacterial restriction/modification system. It provides very useful background knowl General Cloning Protocols Large Scale Preps:(See Large scale plsasmid prep protocol for more details)Cultures: Inoculate a 5 mL LB/Amp (50 - 100 µg/mL) culture in early a 重组DNA的分离、克隆与测序实验手册-5 C. Random fragment end-repair, size selection, and phosphorylationSince both sonicated and nebulized DNA fragments usually contain single-stranded end Cloning PCR products using TA vectors Cloning PCR products using TA vectorsby Paul N. Hengen, Ph.D.*Methods and reagents is a unique monthly column that highlights current discussion Genotyping Transgenic Rodents by PCR Genotyping Transgenic Rodents byPCRThis is how we test mice and rats for the presence of the transgene by PCR. It is provided for those investig Competitive RT-PCR Strategy for Quantitative Evaluation -1 Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type IQuantizatio ES Cell Culture and Manipulation MediaHigh glucose DMEM (-pyruvate, -glutamine)20% Heat-inactivated Fetal calf serum (can vary by cell type, be sure!!)1X l-glutamine1X Penicillin/stre Nucleofection This is an extract of the Amaxa Biosystems protocol Vs. 09-2005 optimized for use with the UC06 cell line. It is suggested that you try all 5 programs RNA实验方法-2 13.Prehybridization mix (add in order listed at 5 mL/100 cm2membrane)Conc.5 mL10 mL15 mLx mLStocks5x500 礚100 FISH protocols for Drosophila-2 3.Methods3.1 RNA Probe Preparation1. Different strategies can be used to prepare template DNA for synthesizing antisense RNA p 定量RT-PCR （Quantitative RT-PCR） Application:Quantitative RT-PCRis used to quantify mRNA in both relative and absolute terms. It can be applied for the quantification of m Roche公司的RNase Protection Assay (RPA) protocol Roche公司的RNase Protection Assay (RPA) Using DIG-Labeled RNA Probes下载网址：http://www.roche-applied-science.com/PROD_INF/BIOCHEMI/no1_03/PDF/p22_23.pdf还有一份 RNA实验方法-3 Part III: HybridizationTurn heatblock on to 95C.For samples in water or ethanol, dry down appropriate amount of RNA, and include a tube with 1ul of tR RNAi载体pSIREN-DNR Vector Restriction Map and Cloning Site of the RNAi-Ready pSIREN-DNR Vector.Unique restriction sites are in bold. RNAi-Ready pSIREN-DNR is provided as Expression Library Screening (Procaryotic) Using AP-Fusion Proteins Outline:Bacteriophage lambda is a linear double stranded DNA, approximately 50 kB in size. The two sticky ends help the phage to recircularize after e